Mutant interleukin-2 (IL-2) polypeptides
AssigneeMassachusetts Institute of Technology
InventorsK. Dane Wittrup, Balaji M. Rao, Douglas A. Lauffenburger
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Abstract
The present invention relates to IL-2 mutants with increased affinity for the IL-2 alpha-receptor subunit (IL-2Rα). The invention thus includes IL-2 mutants with improved biological potency. The invention also includes methods for directed evolution of IL-2α using yeast surface display to generate mutants with increased affinity for IL-2Rα.
Claims18
A mutant interleukin-2 (IL-2) polypeptide comprising an amino acid sequence that is at least 80% identical to SEQ ID NO:2 and that binds an IL-2 receptor α subunit (IL-2Rα) with an affinity that is greater than the affinity with which the polypeptide represented by SEQ ID NO:3 binds the IL-2Rα,wherein the amino acid sequence of the mutant IL-2 polypeptide differs from SEQ ID NO:2 by virtue of containing alanine at position 69 rather than valine.
Dependencies are inferred from the claim text.