US 7,567,870 B1Granted

Multiparameter analysis for predictive medicine

AssigneeInstitute for Systems Biology

InventorsLeroy Hood, Andrew F. Siegel

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Abstract

The invention provides a method of determining a comparative expression profile in an individual by comparing the expression levels of a sample of molecules in a population of molecules in a specimen from the individual with a health-associated reference expression region of the sample of molecules, wherein expression levels within the health-associated reference expression region indicate a reference expression profile and wherein expression levels outside the health-associated reference expression region indicate a perturbed expression profile. The invention also provides methods of diagnosing a disease or a health state in an individual by comparing the expression level of a sample of molecules in a specimen from the individual with a health-associated reference expression region of the sample of molecules.

Claims79

Dependent claims

A method of determining a comparative expression profile in an individual, comprising:

(a) creating a multidimensional space of n dimensions, wherein n represents the number of molecules being analyzed in a specimen from each individual in a population of reference individuals and wherein said multidimensional space contains n axes, each of said axes relating to the expression level of a molecule of said n molecules;
(b) determining a multidimensional coordinate point for each individual in said population of reference individuals, wherein said multidimensional coordinate point is representative of the expression levels of said n molecules in the individual;
(c) determining in said multidimensional space a health-associated reference expression region for said multidimensional coordinate points determined for said population of reference individuals;
(d) contacting a specimen from an individual with a target;
(e) determining the expression levels of a sample of n molecules in a population of molecules in said specimen from said individual;
(f) determining a multidimensional coordinate point representative of the expression levels of said sample of n molecules in said specimen from said individual determined in step (e);
(g) comparing said multidimensional coordinate point determined in step (f) to said health-associated reference expression region determined in step (c);
(h) determining if said multidimensional coordinate point is within or outside said health-associated reference expression region, wherein said multidimensional coordinate point within said health-associated reference expression region indicates a reference expression profile and wherein said multidimensional coordinate point outside said health-associated reference expression region indicates a perturbed expression profile, wherein said perturbed expression profile indicates a disease state in said individual;
and
(i) providing an output of said expression profile determined in step (h) to a user.

7 more elements

A method of diagnosing a health state, comprising:

(a) creating a multidimensional space of n dimensions, wherein n represents the number of molecules being analyzed in a specimen from each individual in a population of reference individuals and wherein said multidimensional space contains n axes, each of said axes relating to the expression level of a molecule of said n molecules;
(b) determining a multidimensional coordinate point for each individual in said population of reference individuals, wherein said multidimensional coordinate point is representative of the expression levels of said n molecules in the individual;
(c) determining in said multidimensional space a health-associated reference expression region for said multidimensional coordinate points determined for said population of reference individuals;
(d) contacting a specimen from an individual with a target;
(e) determining the expression levels of a sample of n molecules in a population of molecules in said specimen from said individual;
(f) determining a multidimensional coordinate point representative of the expression levels of the n molecules determined in step (e);
(g) comparing said multidimensional coordinate point determined in step (f) with said health-associated reference expression region determined in step (c);
(h) determining if said multidimensional coordinate point is within or outside said health-associated reference expression region, wherein said multidimensional coordinate point within said health-associated reference expression region indicates a reference health state and wherein said multidimensional coordinate point outside said health-associated reference expression region indicates a disease state in said individual;
and
(i) providing an output of said health state determined in step (h) to a user.

7 more elements

A method of diagnosing a health state in an individual, comprising:

(a) creating a multidimensional space of n dimensions, wherein n represents the number of molecules being analyzed in a specimen from each individual in a population of reference individuals and wherein said multidimensional space contains n axes, each of said axes relating to the expression level of a molecule of said n molecules;
(b) determining a multidimensional coordinate point for each individual in said population of reference individuals, wherein said multidimensional coordinate point is representative of the expression levels of said n molecules in the individual;
(c) determining in said multidimensional space a health-associated reference expression region for said multidimensional coordinate points determined for said population of reference individuals;
(d) contacting a specimen from an individual with a target;
(e) determining the expression levels of a sample of n molecules in a population of molecules in said specimen from said individual by measuring the expression level of said n molecules in said specimen;
(f) determining a multidimensional coordinate point representative of the expression levels of the n molecules determined in step (e);
(g) comparing said multidimensional coordinate point determined in step (f) with said health-associated reference expression region determined in step (c);
(h) determining if said multidimensional coordinate point is within or outside said health-associated reference expression region, wherein said multidimensional coordinate point within said health-associated reference expression region indicates a reference health state and wherein said multidimensional coordinate point outside said health-associated reference expression region indicates a perturbed health state, thereby diagnosing the health state of said individual;
and
(i) providing an output of said health state determined in step (h) to a user.

7 more elements

A method of diagnosing a health state in an individual, comprising:

(a) creating a multidimensional space of n dimensions, wherein n represents the number of molecules being analyzed in a specimen from each individual in a population of reference individuals and wherein said multidimensional space contains n axes, each of said axes relating to the expression level of a molecule of said n molecules;
(b) determining a multidimensional coordinate point for each individual in said population of reference individuals, wherein said multidimensional coordinate point is representative of the expression levels of said n molecules in the individual;
(c) determining in said multidimensional space a health-associated reference expression region for said multidimensional coordinate points determined for said population of reference individuals;
(d) contacting a leukocyte specimen from an individual with a target;
(e) determining the expression levels of a sample of n molecules in a population of molecules in said leukocyte specimen by measuring the expression level of said n molecules in said specimen;
(f) determining a multidimensional coordinate point representative of the expression levels of the n molecules determined in step (e);
(g) comparing said multidimensional coordinate point determined in step (f) with said health-associated reference expression region determined in step (c);
(h) determining if said multidimensional coordinate point is within or outside said health-associated reference expression region, wherein expression levels within said health-associated reference expression region indicates a reference health state and wherein expression levels outside said health-associated reference expression region indicates a perturbed health state, thereby diagnosing the health state of said individual:
and
(i) providing an output of said health state determined in step (h) to a user.

7 more elements

Dependencies are inferred from the claim text.

Facts

Application: 09/724,898Filed 28 Nov 2000 · us provisional application
Priority: 31 Jul 2000
Granted: 28 Jul 2009
Term ends: Not estimatedThe source records don’t support an estimate.

Classification3 codes

G01N 33/6803
Involving proteins, peptides or amino acids: general methods of protein analysis not limited to specific proteins or families of proteins
G01N 2800/60
Detection or diagnosis of diseases: complex ways of combining multiple protein biomarkers for diagnosis
G01N
Investigating or analysing materials by determining their chemical or physical properties