Labelled nucleotides
AssigneeIllumina Cambridge Limited
InventorsShankar Balasubramanian, Colin Barnes, Xiaohai Liu, John Milton, Harold Swerdlow, Xiaolin Wu
Life of this patent
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Abstract
Nucleosides and nucleotides are disclosed that are linked to detectable labels via a cleavable linker group.
Claims15
A method of labeling a nucleic acid molecule, the method comprising incorporating into the nucleic acid molecule a nucleotide or nucleoside molecule, wherein the nucleotide or nucleoside molecule has a base that is linked to a detectable label via a cleavable linker and the nucleotide or nucleoside molecule has a ribose or deoxyribose sugar moiety, wherein the ribose or deoxyribose sugar moiety comprises a protecting group attached via the 2′ or 3′ oxygen atom, and said protecting group can be modified or removed to expose a 3′ OH group and the protecting group comprises an azido group.
A method of labeling a nucleic acid molecule, the method comprising incorporating into the nucleic acid molecule a nucleotide or nucleoside molecule, wherein the nucleotide or nucleoside molecule has a base that is linked to a detectable label via a cleavable linker and the nucleotide or nucleoside molecule has a ribose or deoxyribose sugar moiety, wherein the ribose or deoxyribose sugar moiety comprises a protecting group attached via the 2′ or 3′ oxygen atom, and said protecting group can be modified or removed to expose a 3′ OH group and the protecting group and cleavable linker are removable under identical conditions.
A method of labeling a nucleic acid molecule, the method comprising:
- a) incorporating into the nucleic acid molecule a nucleotide or nucleoside molecule, wherein the nucleotide or nucleoside molecule has a base that is linked to a detectable label via a cleavable linker, wherein the nucleotide or nucleoside molecule has a ribose or deoxyribose sugar moiety that comprises a protecting group attached via the 2′ or 3′ oxygen atom, and said protecting group can be modified or removed to expose a 3′ OH group and the protecting group comprises an azido group;
- b) detecting the detectable label and cleaving the cleavable linker, wherein the protected hydroxyl functionality and the protecting group are cleavable under identical conditions;
- and
- c) repeating steps a) and b) at least once.
A method of labeling a nucleic acid molecule, the method comprising:
- a) incorporating into the nucleic acid molecule a nucleotide or nucleoside molecule, wherein the nucleotide or nucleoside molecule has a base that is linked to a detectable label via a cleavable linker, wherein the nucleotide or nucleoside molecule has a ribose or deoxyribose sugar moiety that comprises a protecting group attached via the 2′ or 3′ oxygen atom, and said protecting group can be modified or removed to expose a 3′ OH group and the protecting group comprises an allyl moiety;
- b) detecting the detectable label and cleaving the cleavable linker, wherein the protected hydroxyl functionality and the protecting group are cleavable under identical conditions;
- and
- c) repeating steps a) and b) at least once.
Dependencies are inferred from the claim text.