Mutant 2,5-diketo-L-gluconic acid reductases
AssigneeGenencor International, Inc.
InventorsMark Donnelly, William H. Eschenfeldt, Jonathan Trent
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Abstract
Described herein are novel nucleic acids, proteins and methods that can be used to provide new catalysts with desirable traits for industrial processes. In particular, novel reductases isolated from the environment using PCR methods are described.
Claims12
An isolated polypeptide having at least 95% sequence identity to the amino acid sequence of SEQ ID NO:10 and comprising a Q residue at a position corresponding to position 232 or position 238 of SEQ ID NO:10, wherein said polypeptide has 2,5-diketo-D-gluconic acid reductase activity.
A variant of a 2,5-diketo-D-gluconic acid reductase (DKGR) enzyme having the amino acid sequence of SEQ ID NO:10, wherein said variant consists of a substitution at the Q residue of position 232 or position 238 of SEQ ID NO:10 and said variant has DKGR activity.
Dependencies are inferred from the claim text.