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US 7,563,600 B2Granted
Microarray synthesis and assembly of gene-length polynucleotides
AssigneeCombiMatrix Corporation
InventorAndrew V. Oleinikov
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Abstract
There is disclosed a process for in vitro synthesis and assembly of long, gene-length polynucleotides based upon assembly of multiple shorter oligonucleotides synthesized in situ on a microarray platform. Specifically, there is disclosed a process for in situ synthesis of oligonucleotide fragments on a solid phase microarray platform and subsequent, “on device” assembly of larger polynucleotides composed of a plurality of shorter oligonucleotide fragments.
Claims4
A composition for the manufacture of a polynucleotide sequence comprising:
- a first oligonucleotide comprising at its 5′ end a sequence region of about 10 to about 50 bases that is the same as a sequence region at the 3′ end of another oligonucleotide in the composition;
- a second oligonucleotide comprising at its 3′ end a sequence region of about 10 to about 50 bases that is the same as a sequence region at the 5′ end of another oligonucleotide in the composition;
- and
- a plurality of oligonucleotides wherein each oligonucleotide of the plurality comprises a first sequence region of about 10 to about 50 bases at its 5′ end that is the same as a sequence region at the 3′ end of another oligonucleotide in the composition and a second distinct sequence region of about 10 to about 50 bases at its 3′ end that is the same as a sequence region at the 5′ end of a different oligonucleotide in the composition;
- wherein the oligonucleotides of the composition are attached to a solid or porous surface and wherein the oligonucleotides of the composition together comprise the polynucleotide sequence.
2 more elements
Dependencies are inferred from the claim text.