Enzymes for the preparation of amides
AssigneeLonza Ltd.
InventorsKaren Tracey Robins, Toru Nagasawa
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Abstract
A novel biotechnological process for the preparation of nitriles, starting from amides, is described. Micro-organisms of the genus Amycolatopsis, Actinomadura or Rhodococcus are employed for this process.
Claims8
An enzyme having nitrile hydratase activity obtained from a microorganism of the genus Amycolatopsis;
- wherein the enzyme has the following properties:
- (a) a pH optimum of pH 6.5±1.0;
- (b) a temperature optimum between 35 and 40.degree. C. at a pH of 7.0;
- and
- (c) a KM value for the substrate 3-cyanopyridine of 41.7 mM±7.7 mM.
A nitrile hydratase enzyme obtained from a microorganism from which the enzyme is obtained is selected from the group consisting of Amycolatopsis sp. NA40, deposited in the Deutschen Sammlung von Mikroorganismen und Zellkulturer GmbH and having accession number DSM 11617 and Amycolatopsis sp. NE31, deposited in the Deutschen Sammlung von Mikroorganismen und Zellkulturer GmbH and having accession number DSM 11616.
Dependencies are inferred from the claim text.