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US 7,553,615 B2Granted
Compounds, methods, complexes, apparatuses and uses relating to stabile forms of NAD/NADH
AssigneeRoche Diagnostics Operations, Inc.
InventorsDieter Heindl, Joachim Hoenes, Carina Horn, Piet Herdewijn
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Abstract
The invention concerns stable nicotinamide adenine dinucleotide (NAD/NADH) and nicotinamide adenine dinucleotide phosphate (NADP/NADPH) derivatives, enzyme complexes of these derivatives and their use in biochemical detection methods and reagent matrices.
Claims11
A test element for determining an analyte, comprising (i) a nicotinamide coenzyme-dependent enzyme or a substrate for such an enzyme, and (ii) a coenzyme comprising a compound of the following general formula (I) or a salt or a reduced form thereof:
- in which
- A=adenine or an analog thereof,
- T=O or S,
- U=OH, SH, BH3—, or BCNH2—,
- V=OH or a phosphate group,
- W=COOR, CON(R)2, COR, or CSN(R)2, in which R═H or C1-C2-alkyl
- X1, X2=O, CH2, CHCH3, C(CH3)2, NH, or NCH3,
- Y=NH, S, O, or CH2,
- Z= a saturated or unsaturated carbocyclic or heterocyclic six-membered ring, and a residue C(R4)2 bound to the six-membered ring and to X2, where R4=H, F, or CH3,
- wherein the six-membered ring and the pyrimidine residue are not linked by a glycosidic bond.
7 more elements
Dependencies are inferred from the claim text.