Plant producing hyaluronic acid
AssigneeToyo Boseki Kabushiki Kaisha
InventorsShigeo Shibatani, Shuhei Misawa, Izumi Ihara, Hiroaki Kitazawa
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Abstract
The invention relates to a method of producing hyaluronic acid including (1) a step of transforming a plant cell using an expression recombinant vector including (i) a DNA encoding hyaluronic acid synthase or (ii) a DNA encoding a polypeptide having an amino acid sequence having one or more amino acid deletions, substitutions, additions or insertions in an amino acid sequence of the hyaluronic acid synthase and having an activity of synthesizing the hyaluronic acid, (2) a step of growing a transformant obtained by transformation, and (3) a step of separating the hyaluronic acid produced by the transformant.
Claims11
A method of producing hyaluronic acid comprising
- (1) a step of transforming a plant cell using an expression recombinant vector comprising
- (i) a DNA encoding hyaluronic acid synthase or
- (ii) a DNA encoding a polypeptide having an amino acid sequence of the hyaluronic acid synthase,
- (2) a step of growing a transformant obtained by transformation of step (1), and
- (3) a step of separating the hyaluronic acid produced by the transformant, wherein the hyaluronic acid synthase synthase is derived from a chlorella virus.
A method of producing hyaluronic acid comprising
- (1) a step of transforming a plant using an expression recombinant vector comprising
- (i) a DNA encoding hyaluronic acid synthase or
- (ii) a DNA encoding a polypeptide having an amino acid sequence of the hyaluronic acid synthase,
- (2) a step of growing a transformant obtained by transformation of step (1), and
- (3) a step of separating the hyaluronic acid produced by the transformant, wherein the hyaluronic acid is derived from a chlorella virus.
A method of making a transformed plant cell having an ability of producing hyaluronic acid comprising
- a step of transforming a plant cell using an expression recombinant vector comprising
- (i) a DNA encoding hyaluronic acid synthase or
- (ii) a DNA encoding a polypeptide having an amino acid sequence of the hyaluronic acid synthase,
- wherein the hyaluronic acid synthase is derived from a chlorella virus.
A method of making a transformed plant having an ability of producing hyaluronic acid comprising
- a step of transforming a plant cell using an expression recombinant vector comprising
- (i) a DNA encoding hyaluronic acid synthase or
- (ii) a DNA encoding a polypeptide having an amino acid sequence of the hyaluronic acid synthase,
- wherein the hyaluronic acid synthase is derived from a chlorella virus.
A transformed plant cell having an ability of producing hyaluronic acid, obtained by transforming a plant cell using an expression recombinant vector comprising
- (i) a DNA encoding hyaluronic acid synthase or
- (ii) a DNA encoding a polypeptide having an amino acid sequence of the hyaluronic acid synthase,
- wherein the hyaluronic acid synthase is derived from a chlorella virus.
A transformed plant, or a progeny thereof or an organ thereof or a tissue thereof, having the ability of producing hyaluronic acid, wherein said plant is obtained by transforming a plant using an expression recombinant vector comprising
- (i) a DNA encoding hyaluronic acid synthase or
- (ii) a DNA encoding a polypeptide having an amino acid sequence of the hyaluronic acid synthase,
- wherein the hyaluronic acid synthase is derived from a chlorella virus.
Dependencies are inferred from the claim text.