Methods for preparing and using a zinc complex

A method for capturing a substance having an anionic substituent which comprises contacting a substance having an anionic substituent with a zinc complex such that said substance binds to the zinc complex, the zinc complex being represented by a formula (I):

• wherein each R is the same or different from each other and each R represents H;
• an alkyl group having 1 to 16 carbon atoms;
• an acyl group, a carboxyalkyl group, an acylalkyl group, a carbamoylalkyl group, a cyanoalkyl group, a hydroxyalkyl group, an aminoalkyl group or a haloalkyl group, wherein an alkyl portion of said acyl group, said carboxyalkyl group, said acylalkyl group, said carbamoylalkyl group, said cyanoalkyl group, said hydroxyalkyl group, said aminoalkyl group and said haloalkyl group has 1 to 16 carbon atoms;
• a carboxyl group;
• a carbamoyl group;
• a hydroxyl group;
• a cyano group;
• an amino group;
• or a halogeno group,
• and the anionic substituent is selected from the group consisting of —COO−, —OPO32−, —SO3− and —Cl−.

A method for deactivating a phosphorylated substance comprising contacting the substance with a zinc complex represented by a formula (I):

• wherein each R is the same or different from each other and each R represents H;
• an alkyl group having 1 to 16 carbon atoms;
• an acyl group, a carboxyalkyl group, an acylalkyl group, a carbamoylalkyl group, a cyanoalkyl group, a hydroxyalkyl group, an aminoalkyl group or a haloalkyl group, wherein an alkyl portion of said acyl group, said carboxyalkyl group, said acylalkyl group, said carbamoylalkyl group, said cyanoalkyl group, said hydroxyalkyl group, said aminoalkyl group and said haloalkyl group has 1 to 16 carbon atoms;
• a carboxyl group;
• a carbamoyl group;
• a hydroxyl group;
• a cyano group;
• an amino group;
• or a halogeno group,
• and the phosphorylated substance has an anionic substituent selected from the group consisting of —COO−, —OPO32−, —SO3− and —Cl−.

A method for inhibiting a phosphatase comprising contacting a phosphorylated site of a protein or a nucleotide with a zinc complex represented by the formula (I):

• wherein each R is the same or different from each other and each R represents H;
• an alkyl group having 1 to 16 carbon atoms;
• an acyl group, a carboxyalkyl group, an acylalkyl group, a carbamoylalkyl group, a cyanoalkyl group, a hydroxyalkyl group, an aminoalkyl group or a haloalkyl group, wherein an alkyl portion of said acyl group, said carboxyalkyl group, said acylalkyl group, said carbamoylalkyl group, said cyanoalkyl group, said hydroxyalkyl group, said aminoalkyl group and said haloalkyl group has 1 to 16 carbon atoms;
• a carboxyl group;
• a carbamoyl group;
• a hydroxyl group;
• a cyano group;
• an amino group;
• or a halogeno group, and
• the phosphorylated site of a protein or a nucleotide is a phosphate-bound site of a protein or a nucleotide.

A method for carrying out a mass spectrometry comprising the mass spectrometry being carried out in the presence of a zinc complex represented by a formula (I):

• wherein each R is the same or different from each other and each R represents H;
• an alkyl group having 1 to 16 carbon atoms;
• an acyl group, a carboxyalkyl group, an acylalkyl group, a carbamoylalkyl group, a cyanoalkyl group, a hydroxyalkyl group, an aminoalkyl group or a haloalkyl group, wherein an alkyl portion of said acyl group, said carboxyalkyl group, said acylalkyl group, said carbamoylalkyl group, said cyanoalkyl group, said hydroxyalkyl group, said aminoalkyl group and said haloalkyl group has 1 to 16 carbon atoms;
• a carboxyl group;
• a carbamoyl group;
• a hydroxyl group;
• a cyano group;
• an amino group;
• or a halogeno group.

A method for carrying out an electrophoresis comprising the electrophoresis being carried out in the presence of a zinc complex represented by a formula (I):

• wherein each R is the same or different from each other and each R represents H;
• an alkyl group having 1 to 16 carbon atoms;
• an acyl group, a carboxyalkyl group, an acylalkyl group, a carbamoylalkyl group, a cyanoalkyl group, a hydroxyalkyl group, an aminoalkyl group or a haloalkyl group, wherein an alkyl portion of said acyl group, said carboxyalkyl group, said acylalkyl group, said carbamoylalkyl group, said cyanoalkyl group, said hydroxyalkyl group, said aminoalkyl group and said haloalkyl group has 1 to 16 carbon atoms;
• a carboxyl group;
• a carbamoyl group;
• a hydroxyl group;
• a cyano group;
• an amino group;
• or a halogeno group.

A method for carrying out a nuclear magnetic resonance comprising the nuclear magnetic resonance being carried out in the presence of a zinc complex represented by a formula (I):

• wherein each R is the same or different from each other and each R represents H;
• an alkyl group having 1 to 16 carbon atoms;
• an acyl group, a carboxyalkyl group, an acylalkyl group, a carbamoylalkyl group, a cyanoalkyl group, a hydroxyalkyl group, an aminoalkyl group or a haloalkyl group, wherein an alkyl portion of said acyl group, said carboxyalkyl group, said acylalkyl group, said carbamoylalkyl group, said cyanoalkyl group, said hydroxyalkyl group, said aminoalkyl group and said haloalkyl group has 1 to 16 carbon atoms;
• a carboxyl group;
• a carbamoyl group;
• a hydroxyl group;
• a cyano group;
• an amino group;
• or a halogeno group.

A method for carrying out a chromatography comprising the chromatography being carried out in the presence of a zinc complex represented by a formula (I):

• wherein each R is the same or different from each other and each R represents H;
• an alkyl group having 1 to 16 carbon atoms;
• an acyl group, a carboxyalkyl group, an acylalkyl group, a carbamoylalkyl group, a cyanoalkyl group, a hydroxyalkyl group, an aminoalkyl group or a haloalkyl group, wherein an alkyl portion of said acyl group, said carboxyalkyl group, said acylalkyl group, said carbamoylalkyl group, said cyanoalkyl group, said hydroxyalkyl group, said aminoalkyl group and said haloalkyl group has 1 to 16 carbon atoms;
• a carboxyl group;
• a carbamoyl group;
• a hydroxyl group;
• a cyano group;
• an amino group;
• or a halogeno group.