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AssigneeEnzo Life Sciences, Inc.
InventorsElazar Rabbani, Jannis G. Stavrianopoulos, James J. Donegan, Jack Coleman, Dakai Liu
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This invention provides for compositions for use in real time nucleic acid detection processes. Such real time nucleic acid detection processes are carried out with energy transfer elements attached to nucleic acid primers, nucleotides, nucleic acid probes or nucleic acid binding agents. Real time nucleic acid detection allows for the qualitative or quantitative detection or determination of single-stranded or double-stranded nucleic acids of interest in a sample. Other processes are provided by this invention including processes for removing a portion of a homopolymeric sequence, e.g., poly A sequence or tail, from an analyte or library of analytes. Compositions useful in carrying out such removal processes are also described and provided.
A chimeric nucleic acid construct comprising at least two segments, the first segment comprising five or more universal bases and the second segment comprising a nucleic acid template for synthesizing a discrete nucleic acid sequence wherein said nucleic acid construct is blocked at the 3′ end thereby rendering said construct incapable of strand extension.
A composition comprising a set of chimeric nucleic acid constructs, each such construct comprising at least three segments, the first segment comprising four or more universal bases, the second segment comprising permutations of from one to eight nucleotides, the third segment comprising a template for synthesizing a desirable nucleic acid sequence,
A chimeric nucleic acid construct that comprises at least two segments,
A chimeric nucleic acid construct that comprises at least two nucleic acid segments, wherein the first segment and the second segment each comprise sequences complementary to different portions of a homopolymeric sequence in a ribonucleic acid of interest,
Dependencies are inferred from the claim text.