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US 7,547,765 B2Granted
Branched polyalkylene glycols
AssigneeKyowa Hakko Kirin Co., Ltd.
InventorsMotoo Yamasaki, Toshiyuki Suzawa, Tatsuya Murakami, Noriko Sukurai, Kinya Yamashita, Mayumi Mukai, Takashi Kuwabara
Life of this patent
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Abstract
The present invention provides a branched polyalkylene glycol wherein three or more single-chain polyalkylene glycols and a group having reactivity with an amino acid side chain, the N-terminal amino group or the C-terminal carboxyl group in a polypeptide or a group convertible into the group having reactivity are bound; and a physiologically active polypeptide modified with the branched polyalkylene glycol.
Claims15
A branched polyalkylene glycol represented by formula (I):
- (R1—Mn—X1)mL(X2—X3—R2)q (I)
- {wherein L is
- M represents OCH2CH2, OCH2CH2CH2, OCH(CH3)CH2, (OCH2CH2)r—(OCH2CH2CH2)s (in which r and s, which may be the same or different, each represent an arbitrary positive integer) or (OCH2CH2)ra—[OCH(CH3)CH2]sa (in which ra and sa have the same meanings as the above r and s, respectively);
- n represents an arbitrary positive integer;
- m represents an integer of 3 or more;
- q represents an integer of 1 to 3;
- R1 represents a hydrogen atom, lower alkyl or lower alkanoyl;
- R2 represents a group having reactivity with an amino acid side chain, the N-terminal amino group or the C-terminal carboxyl group in a polypeptide or a group convertible into the group having reactivity;
- X1 represents R4—NH—C(═O)—R5 [in which R4 represents a bond, alkylene or O(CH2)tc (in which tc represents an integer of 1 to 8) and R5 represents a bond;
- X2 represents a bond, O or (CH2)teO (in which te has the same meaning as the above tc);
- X3 represents a bond or alkylene;
- and
- three or more R1—Mn—X1's may be the same or different, and when two or three X2—X3—R2's are present (when q is 2 or 3), they may be the same or different}.
10 more elements
Dependencies are inferred from the claim text.