Method for identifying substances useful for treating inflammation using the response element to the ikbalpha ROR receptor

A method of screening test substances to identify candidate substances useful for decreasing inflammation, wherein said method comprises the following steps:

• a) placing each test substance in contact with a nucleic acid construct containing at least one ROR response element site of the IKBα gene promoter, under conditions allowing each test substance to bind to said at least one response element site, b) identifying the test substances which effectively bind to the response element, c) selecting the test substances that bind to the response element as candidate substances useful for decreasing inflammation;
• wherein said ROR response element site consists of SEQ ID NO:
• 1.

A method of screening test substances to identify candidate substances useful for treating inflammation, wherein said method comprises the following steps:

• a) placing each test substance in contact with a host cell transformed with a nucleic acid construct containing at least one reporter gene operably linked to a ROR response element site of the IKBα gene promoter or from a promoter containing said at least one ROR response element site, b) measuring the expression of the reporter gene by any suitable means and so identifying the test substances which effectively increase the transcriptional activity from the response element site or from the promoter containing said response element site and potentially bind to the response element, c) selecting the test substances that increase the transcriptional activity from the response element site or from the promoter containing said response element site as candidate substances useful for decreasing inflammation;
• wherein said Retinoid-related Orphan Receptor (ROR) response element site consists of SEQ ID NO:1.