Methods for reducing the formation of by-products in the production of recombinant polypeptides
AssigneeAsubio Pharma Co., Ltd.
InventorsMasayuki Yabuta, Toshihiro Sawano, Yumiko Masuda, Kazuhiro Ohsuye
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Abstract
A method for reducing the formation of a byproduct polypeptide containing an O-acetylserine residue in place of a serine residue by adding at least one of histidine, methionine or glycine to the medium in a method for producing a polypeptide containing a serine residue by culturing transformed cells, and a method for producing a polypeptide containing a serine residue by culturing transformed cells, characterized by reducing the formation of a byproduct polypeptide containing an O-acetylserine residue in place of a serine residue by adding at least one of histidine, methionine or glycine to the medium.
Claims13
A method for producing a polypeptide comprising a serine residue comprising:
- (i) culturing, in a medium, transformed host cells that produce a recombinant atrial natriuretic peptide comprising a serine residue and a byproduct polypeptide comprising an O-acetylserine residue in place of a serine residue in the atrial natriuretic peptide;
- (ii) adding at least 3 g/L methionine and at least one of at least 3 g/L histidine or at least 3 g/L glycine to the medium;
- and
- (iii) reducing the formation of said byproduct polypeptide,
- wherein the formation of said byproduct polypeptide is reduced in an amount greater than or equal to 50% as compared to a control medium with no methionine, histidine, or glycine added.
A method for producing a polypeptide comprising a serine residue comprising:
- (i) culturing, in a medium, transformed host cells that produce a recombinant atrial natriuretic peptide comprising a serine residue and a byproduct polypeptide comprising an O-acetylserine residue in place of a serine residue in the atrial natriuretic peptide;
- (ii) adding at least one of at least 3 g/L histidine or at least 3 g/L glycine to the medium;
- and
- (iii) reducing the formation of said byproduct polypeptide,
- wherein the formation of said byproduct polypeptide is reduced in an amount greater than or equal to 50% as compared to a control medium with no histidine or glycine added.
Dependencies are inferred from the claim text.