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US 7,547,526 B2Granted
Cell concentration and pathogen recovery
AssigneePurdue Research Foundation
InventorsMichael R. Ladisch, Xingya Liu, Amanda C. Stewart, Wan-Tzu Chen, Nathan S. Mosier, Thomas Huang, Jeremiah Bwatwa, Richard Hendrickson
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Abstract
Methods and kits for the isolation of organisms. Such methods and kits are particularly useful for concentrating and recovering viable organisms from food material. The recovered organisms are of sufficient number and purity to allow detection using a biochip device.
Claims17
A method of rapid concentration and recovery of viable microorganisms from a sample, the method comprising:
- (a) providing a microorganism-containing sample, the sample comprising a liquid component having a first volume;
- (b) providing a hollow fiber filter comprising at least one hollow fiber strand, wherein the hollow fiber filter comprises fibers having a length between 1 cm and 25 cm;
- (c) reducing the first volume of the liquid component by applying the sample to the hollow fiber filter, wherein the volume is reduced by at least 1000 times;
- (d) back flushing the microorganisms from the hollow fiber filter to remove the microorganisms from the hollow fiber filter without vortexing and in a buffer having a second volume;
- (e) delivering the second volume and the microorganisms from the hollow fiber filter to a biochip device;
- and
- (f) detecting the presence of viable microorganisms using the biochip device.
4 more elements
A method of rapid concentration and recovery of viable microorganisms from a sample, the method comprising:
- (a) providing a microorganism-containing sample, the sample comprising a liquid component;
- (b) providing a hollow fiber filter, comprising at least one hollow fiber strand, the fiber having an average pore size of less than 0.5 μm and a length of between 1 cm and 25 cm;
- (c) reducing a volume of the liquid component by flowing the sample through the hollow fiber filter, wherein the volume is reduced by at least 1000 times;
- (d) recovering viable microorganisms from the hollow fiber filter without vortexing by back flushing the microorganisms from the hollow fiber filter in a buffer having a reduced volume, the buffer being suitable for delivery to a biochip device;
- (e) flowing the buffer containing the recovered viable microorganisms into the biochip device;
- and
- (f) detecting the presence of the viable microorganisms using the biochip device.
4 more elements
Dependencies are inferred from the claim text.