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US 7,547,522 B2Granted
Method to enrich for α(1,3)-galactosyltransferase null pig cells
AssigneeImmerge Biotherapeutics, Inc.
InventorRobert J. Hawley
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Abstract
The invention relates to the genetic manipulation of non-human animals. More particularly, the invention relates to genetic manipulation of non-human animals to be used for xenotransplantation. The invention provides a method of selecting GGTA 1 null cells, a viable GGTA 1 null swine, methods for making such swine, and methods of using cells, tissues and organs of such swine for xenotransplantation.
Claims15
A method of selecting GGTA1 null cells comprising the steps of:
- (a) obtaining a line of cells obtained from a GGTA1 heterozygous swine or swine fetus;
- (b) enriching the cells for GGTA1 null cells by treating the cells with agents that specifically bind an α(1,3)-galactose epitope and that deplete cells that express the epitope;
- and
- (c) scanning the line for viable GGTA1 null cells.
1 more element
Dependencies are inferred from the claim text.