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US 7,547,518 B2Granted
Method of screening endothelial cells for angiogenic capability
AssigneeBecton, Dickinson and Company
InventorMin Wu
Life of this patent
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Abstract
Provided is a method of screening a primary endothelial cell population for angiogenesis capability comprising: (a) measuring the percentage of cells that are positive for VEGF R2 and CD34, the level of VEGF R2, or measuring the VEGF R2 to VEGF R1 ratio in the population; and (b) selecting those populations where the measured percentage or the measured ratio is over a threshold value.
Claims9
A method of selecting for higher angiogenesis capability among endothelial cells in an endothelial cell population, said method comprising:
- a) staining the endothelial cell population with anti-VEGF R2 and anti-VEGF R1;
- b) measuring the VEGF R2 to VEGF R1 ratio in the stained population;
- and
- c) selecting endothelial cells having a higher VEGF R2 to VEGF R1 ratio wherein the higher the VEGF R2 to VEGF R1 ratio, the higher the capability of the endothelial cells to form tubes and the higher the angiogenic capability of the endothelial cells.
1 more element
Dependencies are inferred from the claim text.