US 7,547,506 B2 · Peptide-based fluorescence polarization assay for detection of antibodies to Mycobacterium bovis

Abstract

The present invention provides an assay for detection of M. bovis-infected animals. A tracer, comprising a peptide of M. bovis protein MPB70 conjugated to a fluorophore, is added to a serum sample from an animal to form a mixture. The fluorescence polarization of the mixture in then measured and compared to the fluorescence polarization of a control. The present invention further provides a tracer for use in fluorescence polarization assay to detect antibodies specific for M. bovis. The tracer comprises a peptide of M. bovis protein MPB70 conjugated to a fluorophore, such that the tracer is able to bind to antibodies specific for M. bovis to produce a detectable change in fluorescence polarization.

Claims9

Dependent claims

A method for detecting M. bovis-infected animals, the method comprising:

adding a tracer to a sample from an animal to form a mixture, wherein the tracer is formed by labeling a polypeptide of M. bovis protein MPB70 with a fluorophore, and wherein the polypeptide consists of an amino acid sequence selected from the group consisting of SEQ. ID NO.:2 and SEQ. ID NO.:6;
measuring the fluorescence polarization of the mixture;
measuring the fluorescence polarization of a control;
comparing the fluorescence polarization of the mixture to the fluorescence polarization of the control;
and
detecting the presence of M. bovis antibodies in the animal from the measured fluorescence polarization of the mixture.

3 more elements

Dependencies are inferred from the claim text.

Facts

Application

10/492,998Filed 31 Oct 2002 · us provisional application

Priority

31 Oct 2001

Published

US 2004/0214250 A128 Oct 2004

Granted

16 Jun 2009

Term ends

Not estimatedThe source records don’t support an estimate.

Classification5 codes

G01N 33/5695
Bacteria: mycobacteria
C07K 14/35
From bacteria: from Mycobacteriaceae
G01N 33/542
With immune complex formed in liquid phase: with steric inhibition or signal modification, e.g. fluorescent quenching
G01N
Investigating or analysing materials by determining their chemical or physical properties

Peptide-based fluorescence polarization assay for detection of antibodies to Mycobacterium bovis

Life of this patent

Peptide-based fluorescence polarization assay for detection of antibodies to Mycobacterium bovis

Life of this patent

Abstract

Claims9