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AssigneeMonsanto Technology LLC
InventorsBalasulojini Karunanandaa, Martha Post-Beittenmiller, Mylavarapu Venkatramesh, Ganesh M. Kishore, Gregory M. Thorne, John R. LeDeaux
Estimating the term…
Disclosed are constructs comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase and at least one other sterol synthesis pathway enzyme. Also disclosed are methods for using such constructs to alter sterol production and content in cells, plants, seeds and storage organs of plants. Also provided are oils and compositions containing altered sterol levels produced by use of the disclosed constructs. Novel nucleotide sequences useful in the alteration of sterol production are also provided. Also provided are cells, plants, seeds and storage organs of plants comprising sequences encoding 3-hydroxy-3methylglutaryl-Coenzyme A reductase, at least one other sterol synthesis pathway enzyme and at least one tocopherol synthesis enzyme.
A recombinant construct comprising:
A recombinant vector comprising operably linked in the 5′ to 3′ direction, a promoter, a DNA sequence encoding a polypeptide having a 3-hydroxy-3-methylglutaryl-Coenzyme A reductase enzyme activity, and a transcription termination signal sequence;
A transformed host cell comprising,
A cell culture comprising transformed host cells according to 12.
A process of increasing the formation of steroid pathway products in a transformed host cell as compared to an otherwise identical non-transformed host cell comprising:
A transgenic plant seed transformed with a DNA segment that encodes a polypeptide having 3-hydroxy-3-methylglutaryl-Coenzyme A reductase activity, a DNA segment that encodes a polypeptide having sterol methyl transferase I enzyme activity, and a DNA segment that encodes a polypeptide having a squalene epoxidase, a sterol C4-demethylase, an obtusifoliol C14α-demethylase, a sterol C5-desaturase, or a sterol methyl transferase II activity, and a promoter suitable for driving expression of said polypeptides in said plant cell, wherein said transgenic plant seed is capable of germinating into a transgenic plant that over-accumulates steroid pathway products relative to a non-transformed plant of the same strain.
Dependencies are inferred from the claim text.