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US 7,544,362 B1Granted
N protein mutants of porcine reproductive and respiratory syndrome virus
AssigneePfizer Inc.
InventorsDongwan Yoo, Changhee Lee, Jay Gregory Calvert, Siao-Kun Welch
Life of this patent
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Abstract
The present invention provides a genetically modified PRRS virus, methods to make it and related polypeptides, polynucleotides and various components. Vaccines comprising the genetically modified virus and polynucleotides are also provided.
Claims19
A composition comprising a PRRS virus infectious agent selected from the group consisting of:
- a) an isolated genetically modified PRRS virus, comprising an N protein which has been modified in the NLS-2 region thereof compared to wild-type sequence, wherein the modification to the NLS-2 region of the N protein is in the pat8 or pat7 motif thereof and wherein the genetically modified PRRS virus is attenuated as a result of said modification to the N protein;
- b) an infectious RNA molecule encoding the genetically modified PRRS virus of a);
- and
- c) an isolated polynucleotide molecule comprising a DNA sequence encoding the infectious RNA molecule of b).
1 more element
A composition comprising a PRRS virus infectious agent selected from the group consisting of:
- a) an isolated genetically modified PRRS virus, comprising an N protein which has been modified in the NLS-1 region thereof compared to wild-type sequence, and wherein the genetically modified PRRS virus is attenuated as a result of said modification to the N protein;
- b) an infectious RNA molecule encoding the genetically modified PRRS virus of a);
- and
- c) an isolated polynucleotide molecule comprising a DNA sequence encoding the infectious RNA molecule of b).
1 more element
A composition comprising a North American PRRS virus infectious agent selected from the group consisting of:
- a) an isolated genetically modified PRRS virus, comprising an N protein which has been modified in the NLS-2 region thereof compared to wild-type sequence, wherein the modification to the NLS-2 region of the N protein is in the pat8 or pat7 motif thereof, and wherein the genetically modified PRRS virus is attenuated as a result of said modification to the N protein;
- b) an infectious RNA molecule encoding the genetically modified PRRS virus of a);
- and
- c) an isolated polynucleotide molecule comprising a DNA sequence encoding the infectious RNA molecule of b).
1 more element
Dependencies are inferred from the claim text.