Microarray having a base cleavable sulfonyl linker
AssigneeCombiMatrix Corporation
InventorsDominic Suciu, Hetian Gao
Life of this patent
Estimating the term…
Abstract
The present invention provides a microarray having base cleavable, sulfonyl-containing linkers and a process to make the microarray. Oligonucleotides of any sequence may be synthesized on the microarray having the cleavable linker. The oligonucleotides may be cleaved and recovered as a pool of oligonucleotides having a three prime phosphate moiety. Specifically, the microarray is an electrode containing microarray, and the oligonucleotides are electrochemically synthesized.
Claims36
A process for forming a microarray having base cleavable sulfonyl linkers comprising:
- (a) providing a plurality of known locations each with a plurality of hydroxyl groups, wherein the density of the known locations is greater than approximately 100 per square centimeter;
- and
- (b) bonding a plurality of sulfonyl amidite moieties to the plurality of hydroxyl groups to form a plurality of cleavable linkers at the plurality of known locations, wherein the cleavable linkers have a linker hydroxyl group and a base-labile cleaving site, whereby a phosphorous-oxygen bond is formed between phosphorous of the sulfonyl amidite moieties and oxygen of the hydroxyl groups.
A microarray having base cleavable sulfonyl linkers comprising:
- (a) a plurality of known locations having a plurality of reacted hydroxyl groups, wherein the density of the known locations is greater than approximately 100 per square centimeter;
- and
- (b) a plurality of reacted sulfonyl amidite moieties bonded to the plurality of reacted hydroxyl groups to form a plurality of cleavable linkers attached to the solid surface, wherein the cleavable linkers have a linker hydroxyl group and a base-labile cleaving site, whereby a phosphorous-oxygen bond is between phosphorous of the reacted sulfonyl amidite moieties and oxygen of the reacted hydroxyl groups.
Dependencies are inferred from the claim text.