Recombinant cyclopentanone monooxygenase [cpmo]
AssigneeNational Research Council of Canada
InventorsHiroaki Iwaki, Yoshie Hasegawa, Peter C. K. Lau
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Abstract
Cyclopentanone 1,2-monooxygenase (CPMO) from Comamonas (previously Pseudomonas) sp. strain NCIMB 9872 carries out the second step of a degradation pathway that allows the bacterium to use cyclopentanol as a sole carbon source for growth. In the present invention there is reported the localization of the CPMO-encoding gene (cpnB) on a 4.3-kb SphI fragment, the determination of its sequence. The 550-amino acid CPMO polypeptide (Mr, 62,111) encoded by the gene was found to have 36.5% identity with the sequence of cyclohexanone 1,2-monooxygenase (CHMO) of Acinetobacter sp. strain NCIMB 9871. The 62-kDa CPMO was expressed in E. coli as an IPTG-inducible protein.
Claims4
A recombinant cyclopentanone monooxygenase (CPMO) having:
- (a) the amino acid sequence as set forth in SEQ ID NO:
- 5;
- or
- (b) the amino acid sequence encoded by the nucleic acid sequence as set forth in SEQ ID NO:
- 8.
Dependencies are inferred from the claim text.