Back to results
US 7,541,166 B2Granted
Sonication to selectively lyse different cell types
AssigneeMicroFluidic Systems, Inc.
InventorsPhillip I. Belgrader, Bob Yuan
Life of this patent
Estimating the term…
Abstract
A sonication apparatus is directed to a microfluidic-based system to automate differential extraction of specific cell types within a mixed sample. The microfluidic-based system includes a sonication module for selective cell lysis, separating means to eliminate centrifugation, high surface area pillar chip modules to purify DNA from a cell lysate, and microfluidic circuitry to integrate the steps in an automated platform.
Claims15
A method of selectively lysing a specific type of cell, the method compnsing:
- a. providing a sample to a self-contained, automated system, the sample includes at least two different cell types, wherein a first cell type is lysed at a first sonication energy;
- b. automatically applying the first sonication energy to the sample by lysing means within the automated system thereby lysing the first cell type to form a first lysate and leaving intact one or more non-lysed cell types;
- c. automatically separating the first lysate from the sample including the intact one or more non-lysed cell types by separating means within the automated system, thereby forming a separated sample including the intact one or more non-lysed cell types;
- d. automatically moving the separated sample to the lysing means by a back-flow capability and microfluidic circuitry within the automated system, wherein the microfluidic circuitry includes the back-flow capability for moving the separated sample from the separating means to the lysing means;
- and
- e. automatically lysing a second cell type from the separated sample by lysing means within the automated system thereby lysing the second cell type to form a second lysate.
3 more elements
Dependencies are inferred from the claim text.