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US 7,541,155 B2Granted
Methods of screening compositions for potential insect control activity
AssigneeTyratech, Inc.
InventorEssam Enan
Life of this patent
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Abstract
The present invention comprises compositions, methods and cell lines related to controlling insects. An embodiment of a composition comprises a plant essential oil and targets at least one receptor of insects chosen from tyramine receptor, Or83b olfactory receptor, and Or43a olfactory receptor, resulting in a change in the intracellular levels of cAMP, Ca2+, or both in the insects.
Claims11
A method of screening a composition for potential insect control activity, comprising:
- providing insect cells expressing olfactory receptor Or83b and a tyramine receptor;
- adding said composition to the cells;
- measuring the binding affinity of said composition to the receptor;
- and
- selecting a composition having an affinity for either of said receptors, wherein said affinity is indicative of potential insect control activity.
2 more elements
A method of screening a composition for potential insect control activity comprising:
- providing insect cells expressing olfactory receptor Or83b and a tyramine receptor;
- adding said composition to the cells;
- extracting intracellular cAMP or Ca2+ from the cells;
- measuring the intracellular cAMP or Ca2+ levels;
- comparing the intracellular cAMP or Ca2+ levels in cells treated with said composition to the intracellular cAMP or Ca2+ levels in untreated cells;
- and
- selecting a composition that causes a change in intracellular cAMP, Ca2+, or both via at least one of said receptors, wherein said change is indicative of potential insect control activity.
4 more elements
A method of screening a composition for potential insect control activity, comprising:
- providing insect cells expressing olfactory receptor 83b and a tyramine receptor;
- adding said composition to the cells;
- measuring a characteristic of the cells in the presence of the composition, wherein the characteristic results from an interaction between the composition and at least one of the said receptors and is indicative of a potential for insect control activity;
- and
- selecting a composition suitable for insect control based on the results of the measuring step.
2 more elements
Dependencies are inferred from the claim text.