US 7,531,715 B1 · Double nuclear transfer method and results thereof

Abstract

A method is described for the production of an animal (offspring) by the process of nuclear transfer. The animal may be produced using donor genetic material in cells taken directly from any stage of an animal, such as an embryo, foetus or adult or from cell cultures established from material from any stage of an animal, such as embryonic, foetal or adult material. The process may be used to introduce genetic modifications into the resultant offspring by genetic manipulation and selection of the cells to act as nuclear donors prior to embryo reconstruction. The present invention provides a method of reconstituting an animal embryo, the process comprising transferring a nucleus into a first oocyte followed by removing and transferring the nucleus from the oocyte to a further oocyte or to an enucleated fertilized zygote.

Claims35

Dependent claims

A method of reconstituting a non-primate mammalian embryo, comprising

(i) transferring a non-primate mammalian donor nucleus from a diploid somatic cell into a first recipient oocyte;
(ii) removing the donor nucleus from the first recipient oocyte;
(iii) providing a second recipient oocyte or enucleated fertilized zygote;
and
(iv) transferring the donor nucleus from the first recipient oocyte into the second recipient oocyte or the enucleated fertilized zygote to obtain a reconstituted non-primate mammalian embryo, wherein the second recipient oocyte is activated prior to, concomitant with or following transfer of the donor nucleus.

2 more elements

Dependencies are inferred from the claim text.

Double nuclear transfer method and results thereof

Life of this patent

Double nuclear transfer method and results thereof

Life of this patent

Abstract

Claims35