Mutations for enhanced tyrosine production
AssigneeMassachusetts Institute of Technology
InventorsGregory Stephanopoulos, Tina Lutke-Eversloh
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Abstract
The present invention provides cells genetically engineered to express mutated chorismate mutase/prephenate dehydrogenase (CM/PDH), the nucleic acid coding for each mutated CM/PDH and similar nucleic acids and methods for utilizing cells expressing mutated CM/PDH to produce tyrosine.
Claims11
A cell engineered to express a chorismate mutase/prephenate dehydrogenase (CM/PDH) protein having at least 95% identity to SEQ ID NO:
- 1, wherein said protein
- a) comprises a mutation in at least one amino acid at positions 95, 96, 180, 190, 324 or 357 of SEQ ID NO:
- 1;
- and
- b) is subject to lower or no feedback inhibition by tyrosine.
An isolated nucleic acid comprising the nucleotide sequence as set forth in SEQ ID NO:
- 26, wherein said nucleic acid encodes a polypeptide involved in tyrosine biosyntheseis.
A cell engineered to express a chorismate mutase/prephenate dehydrogenase (CM/PDH) protein, wherein said protein
- a) has the amino acid sequence as set forth in SEQ ID NO:
- 5;
- and
- b) is subject to lower or no feedback inhibition by tyrosine.
Dependencies are inferred from the claim text.