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AssigneeNovartis Vaccines and Diagnostics, Inc.
InventorsPaul A. Luciw, Dino Dina, Kathelyn Steimer, Ray Sanchez Pescador, Carlos George-Nascimento, Deborah Parkes, Rob Hallewell, Philip J. Barr, Martha Truett
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Polynucleotide sequences are provided for the diagnosis of the presence of retroviral infection in a human host associated with lymphadenopathy syndrome and/or acquired immune deficiency syndrome, for expression of polypeptides and use of the polypeptides to prepare antibodies, where both the polypeptides and antibodies may be employed as diagnostic reagents or in therapy, e.g., vaccines and passive immunization. The sequences provide detection of the viral infectious agents associated with the indicated syndromes and can be used for expression of antigenic polypeptides.
A recombinant DNA construct useful for the expression of a recombinant polypeptide in a cell containing the construct, the construct comprising control sequences which regulate transcription and translation of the recombinant polypeptide in the cell and a coding sequence regulated by the control sequences, wherein the coding sequence comprises a DNA sequence of at least about 27 bp of the pol open reading frame shown in FIG. 4, characterized in that the DNA sequence encodes an antigenic pol polypeptide having a sequence within BstXI (3006) to NdeI (5131) which sites are set forth in FIG. 4, which polypeptide sequence is immunologically non-cross-reactive with HTLV-I and HTLV-II and is reactive with HIV.
A purified polynucleotide of at least about 20 bp of the pol open reading frame shown in FIG. 4 which encodes an antigenic pol polypeptide having a sequence within BstXI (3006) to NdeI (5131) which sites are set forth in FIG. 4, which polypeptide sequence is immunologically non-cross-reactive with HTLV-I and HTLV-II and is reactive with HIV.
Dependencies are inferred from the claim text.