Valve control system for a rotating multiplex fluorescence detection device

A detection device comprising:

• a disk having a holding chamber separated from a process chamber by a valve;
• a motor configured to rotate the disk;
• a laser control system comprising:
• a single laser that outputs electromagnetic energy at a first energy level to determine a position of the disk and at a second energy level to open the valve to permit fluid to flow from the holding chamber to the process chamber, wherein the first energy level is different from the second energy level;
• a sensor that outputs a signal upon detection of the electromagnetic energy at the first energy level;
• and
• a control unit coupled to the sensor, wherein the control unit is responsive to the signal and configured to construct a position map based on the signal that maps a position of the valve relative to a known position of the motor, use the position map to rotate the disk and move the energy source to target the valve, and instruct the energy source to output electromagnetic energy at the second energy level to open the valve.