Back to results
US 7,524,681 B2Granted
Rapid assay for biological substances using FTIR
OwnerAndreas Wolf, Ralf Masuch +1inventors
InventorsAndreas Wolf, Ralf Masuch, Robert Seidel
Life of this patent
Estimating the term…
Abstract
The present invention relates to a method for determining the condition of a biological fluid by recording the IR spectrum of a sample of the biological fluid. To this end, the biological fluid can be examined in its native form. The method of the invention is usable, for example, for detecting pathological conditions in organisms.
Claims22
Method for determining the condition of a biological fluid comprising the steps of
- (a) providing several native samples of the biological fluid, the condition of the biological fluid in each sample being known;
- (b) recording the IR spectra of the native samples of step (a);
- (c) subjecting the IR spectra of step (b) to a multiparameter analytical procedure and selecting the classification parameters that will ensure a reliable assignment of the samples to the known conditions;
- (d) storing the classification parameters obtained in step (c);
- (e) providing a native sample of a biological fluid, the condition of which is unknown;
- (f) recording at least one IR spectrum of the native sample of step (e);
- (g) subjecting the IR spectrum of step (f) to a multiparameter analysis;
- and
- (h) comparing the classification parameters of the IR spectrum of the unknown sample with the classification parameters of the IR spectra of the known samples stored in step (d), wherein the IR spectra in steps (b) and (f) are recorded under non-flowing and stopped conditions after filling the flow-through measuring cell with said sample or with a transport medium for a corresponding reference measurement, wherein the measuring cell has a path length of not more than 30 μm and a path length deviation of less than 1 nm between any two consecutive measurements requiring filling or rinsing of said measuring cell between said consecutive measurements, which filling or rinsing can cause flow- or pressure-induced path length deviations.
6 more elements
Method for preparing a collection of conditions of biological fluids, comprising the steps of
- (a) providing several native samples of biological fluids, the condition of each sample of biological fluid being known;
- (b) recording the IR spectra of the native samples of step (a);
- (c) subjecting the IR spectra of step (b) to a multiparameter analytical procedure and selecting the classification parameters that will ensure a reliable assignment of the samples to the known conditions, and
- (d) storing the classification parameters obtained in step (c),
- wherein the recording of the IR spectra in step (b) is carried out under non-flowing and stopped conditions after filling the flow-through measuring cell with said sample or with a transport medium for a corresponding reference measurement in a measuring cell with a path length of not more than 30 μm and which during the recording of the spectra shows a path length deviation of less than 1 nm between any two consecutive measurements requiring filling or rinsing of said measuring cell between said consecutive measurements, which filling or rinsing can cause flow- or pressure-induced path length deviations.
2 more elements
Method for determining the condition of a biological fluid comprising the steps of
- (e) providing a native sample of a biological fluid, the condition of which is unknown;
- (f) recording an IR spectrum of the native sample of a biological fluid;
- (g) subjecting the IR spectrum of step (f) to a multiparameter analysis;
- and
- (h) comparing the classification parameters of the IR spectrum of the unknown sample with the classification parameters of the IR spectrum of the known sample,
- wherein the IR spectra in step (f) are recorded under non-flowing and stopped conditions after filling the flow-through measuring cell with said sample or with a transport medium for a corresponding reference measurement, with the aid of a measuring cell with a path length of not more than 30 μm and which during the recording of the spectra shows a path length deviation of less than 1 nm between any two consecutive measurements requiring filling or rinsing of said measuring cell between said consecutive measurements, which filling or rinsing can cause flow- or pressure-induced path length deviations.
3 more elements
Dependencies are inferred from the claim text.