# US 7,521,592 B2 · Generation of plants with improved pathogen resistance

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- Number: US 7,521,592 B2
- Document ID: US7521592B2
- Status: Granted
- Kind: B2
- Application number: 10/512,600
- Filed: 2003-04-24
- Published: 2005-09-22
- Granted: 2009-04-21
- Earliest claimed: 2003-04-24
- Assignees: Agrinomics LLC
- Inventors: Nancy Anne Federspiel; Allan Lammers; Xing Liang Liu; Stanley R. Bates; Christina Westerlund; Jonathan R. Fitch
- Web page: https://patentgrep.com/patent/US7521592B2

## CPC

- C12N 15/8279: {for biotic stress resistance, pathogen resistance, disease resistance}
- C07K 14/415: from plants
- C12N 15/8281: {for bacterial resistance}
- C12N 15/8282: {for fungal resistance}
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)

## Abstract

The present invention is directed to plants that display a pathogen resistance phenotype due to altered expression of a PPR2 nucleic acid. The invention is further directed to methods of generating plants with a pathogen resistance phenotype.

## Claims

### Claim 1

A method of producing a plant having increased pathogen resistance, said method comprising:
a) introducing into cells of the plant a plant transformation vector comprising a nucleotide sequence that encodes a Myb polypeptide that confers Peronospora parasitica resistance (PPR2), said Myb polypeptide selected from the group consisting of: the polypeptide sequence of SEQ ID NO:2; a polypeptide sequence having at least 95% sequence identity to SEQ ID NO:2; and an Arabidopsis thaliana polypeptide having at least 85% sequence identity to SEQ ID NO:2, and
b) growing the transformed cells to produce a transgenic plant, wherein said nucleotide sequence is expressed, and said transgenic plant exhibits increased pathogen resistance relative to a control plant.

### Claim 2 (depends on 1)

A transformed plant obtained by the method of claim 1.

### Claim 3 (depends on 2)

A transformed plant part obtained from the plant according to claim 2.

### Claim 4 (depends on 2)

The transformed plant of claim 2, wherein the nucleotide sequence is set forth in SEQ ID NO: 1.

### Claim 5 (depends on 2)

The transformed plant of claim 2, wherein the nucleotide sequence encodes an Arabidopsis thaliana Myb polypeptide having at least 85% sequence identity to SEQ ID NO: 2.

### Claim 6 (depends on 5)

The transformed plant of claim 5, wherein the Arabidopsis thaliana Myb polypeptide has at least 95% sequence identity to SEQ ID NO:2.

### Claim 7

A method of producing a plant having increased pathogen resistance, said method comprising:
a) introducing into cells of the plant a plant transformation vector comprising a nucleotide sequence that encodes or is complementary to a sequence that encodes Peronospora parasitica resistance polypeptide comprising the amino acid sequence of SEQ ID NO: 2, SEQ ID NO: 11, or SEQ ID NO: 12; and
b) growing the transformed cells to produce a transgenic plant, wherein said nucleotide sequence is expressed, and said transgenic plant exhibits increased pathogen resistance relative to a control plant.

### Claim 8 (depends on 7)

A transformed plant produced by the method of claim 7.

### Claim 9 (depends on 8)

The transformed plant of claim 8, wherein the nucleotide sequence encodes SEQ ID NO: 2.

### Claim 10 (depends on 9)

The transformed plant of claim 9, wherein the nucleotide sequence comprises SEQ ID NO: 1.

### Claim 11 (depends on 1)

The method of claim 1, wherein the transformation vector further comprises a constitutive promoter operably linked to the nucleotide sequence.

### Claim 12 (depends on 1)

The method of claim 1, wherein the transformation vector further comprises a pathogen inducible promoter operably linked to the nucleotide sequence.

### Claim 13 (depends on 5)

The transformed plant of claim 5, wherein the nucleotide sequence encodes an Arabidopsis thaliana Myb polypeptide having at least 90% sequence identity to SEQ ID NO: 2.