Back to results
US 7,514,258 B2Granted
Mammalian cultured inner cell mass cell culture using a quiescent cell as nuclear
AssigneeRoslin Institute (Edinburgh)
InventorsKeith Henry Stockman Campbell, Ian Wilmut
Life of this patent
Estimating the term…
Abstract
A method of reconstituting an animal embryo involves transferring the nucleus from a quiescent donor cell into a suitable recipient cell. The donor cell is quiescent, in that it is caused to exit from the growth and division cycle at G1 and to arrest in the G0 state. Nuclear transfer may take place by cell fusion. The reconstituted embryo may then give rise to one or more animals. The invention is useful in the production of transgenic animals as well as non-transgenics of high genetic merit.
Claims9
A method for producing a mammalian cultured inner cell mass cell by nuclear transfer comprising:
- (i) inserting a nucleus of a quiescent mammalian differentiated cell into an enucleated mammalian oocyte of the same species to reconstruct an embryo;
- (ii) culturing the reconstructed embryo;
- and
- (iii) isolating and culturing inner cell mass cells obtained from said cultured, reconstructed embryo to obtain a cultured inner cell mass cell.
1 more element
Dependencies are inferred from the claim text.