Postpartum cells isolated from umbilical cord tissue, and methods of making and using the same
AssigneeEthicon, Inc.
InventorsSanjay Mistry, Anthony J. Kihm, Ian Ross Harris, Alexander M. Harmon, Darin J. Messina, Chin-Feng Yi, Anna Gosiewska
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Abstract
Cells derived from human umbilical cords are disclosed along with methods for their therapeutic use. Isolation techniques, culture methods and detailed characterization of the cells with respect to their cell surface markers, gene expression, and their secretion of trophic factors are described.
Claims26
An isolated cell, wherein said isolated cell is obtained by contacting mammalian umbilical cord tissue substantially free of blood with either dispase or dispase and at least one of hyaluronidase and collagenase and obtaining the cells yielded by said contacting step;
- wherein said isolated cell self-renews and expands its numbers in culture;
- wherein said isolated cell can differentiate into a cell that expresses markers indicative of differentiation into a type selected from the group consisting of neural cells, hepatocytes, adipocytes, pancreatic beta cells, chondrocytes, and cardiomyocytes;
- and wherein said isolated cell does not express CD45 or CD117.
Dependencies are inferred from the claim text.