US 7,504,253 B2 · Nucleic acid encoding proteins involved in protein degradation, products and methods related thereof

Abstract

In accordance with the present invention, there are provided novel Siah-Mediated-Degradation-Proteins (SMDPs) and/or SCF-Complex Proteins (SCPs). Nucleic acid sequences encoding such proteins and assays employing same are also disclosed. The invention SMDPs and/or SCPs can be employed in a variety of ways, for example, for the production of anti-SMDP and/or SCP antibodies thereto, in therapeutic compositions, and methods employing such proteins and/or antibodies for drug screening, functional genomics and other applications. Also provided are transgenic non-human mammals that express the invention protein. Also provided are compositions and methods for targeting the destruction of selected polypeptides in eukaryotic cells based on the ubiquitin-independent mechanism by which ornithine decarboxylase is degraded by the 26S proteasome.

Claims18

Dependent claims

An isolated polynucleotide that comprises a promoter sequence operably linked to a sequence that encodes a chimeric protein that comprises a target-protein binding domain operatively linked to a protein-degradation binding domain of ornithine decarboxylase and a flexible linker sequence [Gly-Gly-Gly-Gly-Ser]3 (SEQ ID NO:

61) between the target-protein binding domain and the protein-degradation binding domain of ornithine decarboxylase, wherein binding of the target-protein binding domain of the chimeric protein to a target protein in a cell induces degradation of the target protein in the cell.

Dependencies are inferred from the claim text.

Nucleic acid encoding proteins involved in protein degradation, products and methods related thereof

Life of this patent

Nucleic acid encoding proteins involved in protein degradation, products and methods related thereof

Life of this patent

Abstract

Claims18