Xylanases, nucleic acids encoding them and methods for making and using them
AssigneeVerenium Corporation
InventorsBrian Steer, Walter Callen, Shaun Healey, Geoff Hazlewood, Di Wu, David Blum, Alireza Esteghlalian
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Abstract
The invention relates to xylanases and to polynucleotides encoding the xylanases. In addition, methods of designing new xylanases and methods of use thereof are also provided. The xylanases have increased activity and stability at increased pH and temperature.
Claims45
An isolated, synthetic or recombinant polypeptide having a xylanase activity comprising:
- (i) an amino acid sequence having at least 90% sequence identity to the amino acid sequence of SEQ ID NO:160, or,
- (ii) an amino acid sequence encoded by a nucleic acid having at least 95% sequence identity to the polynucleotide sequence of SEQ ID NO:159;
- or
- (iii) enzymatically active polypeptide fragments of (i) or (ii).
An isolated, synthetic or recombinant polypeptide having a xylanase activity comprising a polypeptide encoded by a nucleic acid capable of hybridizing under stringent conditions to the complement of the sequence of SEQ ID NO:159, wherein the stringent conditions comprise a wash step comprising a wash in 0.2×SSC at a temperature of about 65° C. for about 15 minutes.
Dependencies are inferred from the claim text.