Back to results
US 7,501,238 B2Granted
Skin substitutes for irritancy testing
OwnerAllen Comer, Lynn Allen-Hoffmann +1inventors
InventorsAllen Comer, Lynn Allen-Hoffmann, Michael Hoffmann
Life of this patent
Estimating the term…
Abstract
The present invention relates to in vitro cultured skin substitutes, preferably to in vitro cultured skin substitutes that have improved barrier function. In particular, the present invention relates to the use of such skin substitutes for irritancy testing. The present invention provides improved methods of screening compounds for irritancy activity, as well for identifying novel irritant responsive genes.
Claims7
A method of screening compounds suspected of being skin irritants, comprising:
- a) providing
- i) a compound suspected of being a skin irritant;
- ii) a skin equivalent comprising a irritant responsive reporter gene construct, wherein said construct comprises a promoter selected from the group consisting of interleukin-8 and interleukin-1α promoters operably linked to a reporter gene;
- and
- b) contacting said skin equivalent with said compound;
- and
- c) measuring the level of gene expression from said reporter gene construct, wherein increased expression from said reporter gene construct is indicative of a response to said irritant.
4 more elements
Dependencies are inferred from the claim text.