Methods and interferon deficient substrates for the propagation of viruses
AssigneeMount Sinai School of Medicine of New York University
InventorsPeter Palese, Adolfo García-Sastre, Robert O'Neil
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Abstract
The present invention relates, to novel methods and substrates for the propagation of viruses. The invention relates to IFN-deficient substrates and methods for propagating viruses in these unconventional substrates. In particular, the invention relates to methods of propagating viruses in immature embryonated eggs, preferably six- to nine-day-old chicken eggs. The methods of the invention are particularly attractive for growing viruses suitable for use in vaccine and pharmaceutical formulations.
Claims32
An interferon deficient cell line containing a genetically engineered attenuated influenza virus, in which the genome of the attenuated influenza virus encodes a truncated NS1 protein composed of between 90 and 130 N-terminal amino acid residues of the NS1 protein of the same or a different influenza virus strain, so that the genetically engineered influenza virus has an impaired interferon antagonist phenotype.
An interferon deficient cell line containing a genetically engineered attenuated influenza virus, in which the genome of the attenuated influenza virus encodes a truncated NS1 protein composed of amino acid residues 1 to 130, amino acid residues 1 to 124, amino acid residues 1 to 120, amino acid residues 1 to 110, amino acid residues 1 to 100, amino acid residues 1 to 99, amino acid residues 1 to 90, amino acid residues 1 to 89, amino acid residues 1 to 70, or amino acid residues 1 to 60 of the NS1 protein of the same or a different influenza virus strain, so that the genetically engineered attenuated influenza virus has an impaired interferon antagonist phenotype.
An interferon deficient cell line containing an attenuated influenza virus, in which the genome of the attenuated influenza virus has an NS1 gene of the same or a different influenza virus strain, wherein the NS1 gene has a mutation such that the attenuated influenza virus has an impaired interferon antagonist phenotype, wherein said virus is not influenza C virus and the interferon deficient cell line is not Vero cells and is not STAT1 (−) cell lines.
Dependencies are inferred from the claim text.