Protein secretion in eukaryotic cells
AssigneesVlaams Interuniversitair Institute voor Biotechnologie vzw, Universiteit Gent
InventorsRoland Contreras, Steven Geysens
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Abstract
The present invention relates to the use of a glucosidase II mutation to increase protein secretion in eukaryotic cells. The present invention relates further to the use of eukaryotic cells, comprising a mutant glucosidase II gene, possibly in combination with the expression of a recombinant α-1,2-mannosidase gene and/or a recombinant N-acetylglucosaminyl-transferase gene, as a host for protein secretion.
Claims4
An improvement in a method for secreting protein from a filamentous fungus cell, the improvement comprising:
- utilizing a glucosidase II mutation to increase protein secretion from the filamentous fungus cell.
An improvement in a method of the type involving using yeast to secrete protein, the improvement comprising:
- using in the method a yeast selected from the group consisting of Kluyveromyces sp., Pichia sp., Hansenula sp. or Schizosaccharomyces pombe, the yeast having a defective glucosidase II as host for protein secretion.
Dependencies are inferred from the claim text.