Stable retrovirus and methods of use
AssigneeThe Board of Trustees of the University of Illinois
InventorsHalong N. Vu, Daniel W. Pack, Joshua Ramsey
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Abstract
The present invention is a mutant retroviral protease which confers an increase in retroviral stability. Retroviruses expressing the instant mutant retroviral protease exhibit at least a 2-fold increase in infectivity half-life as compared to wild-type retrovirus. Unexpectedly, a Gly119Glu mutation in the protease enhances retroviral stability in the presence of various wild-type envelope proteins including wild-type amphotropic, ecotropic and 10A1 murine leukemia viruses. The improved stability of the mutant retrovirus leads to more facile virus production and enhanced infection efficiency.
Claims1
An isolated mutant murine leukemia virus protease comprising an amino acid substitution at position 119 of SEQ ID NO:1.
Dependencies are inferred from the claim text.