Method and kits for preparing multicomponent nucleic acid constructs
AssigneeVectorObjects, LLC
InventorsNone recorded
Life of this patent
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Abstract
The invention provides a highly efficient, rapid, and cost effective method of linking nucleic acid components in a predetermined order to produce a nucleic acid multicomponent construct. The invention further provides nucleic acid components, each nucleic acid component comprising a double stranded nucleic acid molecule having at least one single stranded 5′ or 3′ terminal sequence, the terminal sequence having sufficient complementarity to either a terminal sequence in a separate nucleic acid component or to a sequence in a linking nucleic acid molecule so as to allow for specific annealing of complementary sequences and linkage of the components in a predetermined order. Kits containing reagents required to practice the method of the invention are also provided.
Claims19
A method of obtaining a nucleic acid multicomponent construct having a desirable functionality comprising:
- (a) providing a collection of nucleic acid components, each component comprising at least one genetic element having a known functionality and at least one single-stranded 5′ or 3′ terminal sequence, and
- (b) selecting at least three nucleic acid components, wherein a first nucleic acid component is selected from a category of first nucleic acid components having a common biological utility or functionality, a second nucleic acid component is selected from a category of second nucleic acid components having a common biological utility or functionality, and a third nucleic acid component selected from a category of third nucleic acid component having a common biological utility or functionality, wherein the biological utility or functionality of each of the first, second and third categories is different from the other categories, and
- (c) assembling said at least three nucleic acid components by hybridization of the terminal sequence to either a terminal sequence in another of said at least three nucleic acid components, thereby producing a nucleic acid multicomponent construct with desired functionality.
Dependencies are inferred from the claim text.