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US 7,485,307 B2Granted
Human cystathionine β-synthase variants and methods of production thereof
AssigneeThe Regents of the University of Colorado
InventorsJan P. Kraus, Jana Oliveriusova
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Abstract
Human cystathionine β-synthase variants are disclosed, as well as a method to produce recombinant human cystathionine β-synthase and variants thereof. More particularly, the role of both the N-terminal and C-terminal regions of human CBS has been studied, and a variety of truncation mutants and modified CBS homologues are described. In addition, a method to express and purify recombinant human cystathionine β-synthase (CBS) and variants thereof which have only one or two additional amino acid residues at the N-terminus are described.
Claims17
An isolated nucleic acid molecule comprising:
- a) a first nucleic acid sequence encoding a human cystathionine β-synthase variant spanning from a starting position of amino acid residue 1 of SEQ ID NO:2 to an ending position of one of amino acid residues from 382-532 or 543-551 of SEQ ID NO:2;
- or a homologue thereof comprising an amino acid sequence that is at least 95% identical to said amino acid sequence;
- and
- b) a second nucleic acid sequence linked to the 5′-nucleotide of said first nucleic acid sequence, wherein said second nucleic acid sequence encodes a rhinovirus 3C protease recognition sequence.
1 more element
Dependencies are inferred from the claim text.