Nucleic acid detection cassette and nucleic acid detection device
AssigneeKabushiki Kaisha Toshiba
InventorsJun Okada, Sadato Hongo, Kenji Ooki
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AssigneeKabushiki Kaisha Toshiba
InventorsJun Okada, Sadato Hongo, Kenji Ooki
Estimating the term…
A nucleic acid detection cassette includes a cassette body, a nucleic acid detection region disposed in the cassette body, a first channel disposed in the cassette body, a second channel disposed in the cassette body. The nucleic acid detection region, in which a nucleic acid probe is immobilized, has a reagent inflow port, to which the first channel is connected, and a reagent outflow port, to which the second channel is connected. The nucleic acid detection cassette further includes a reagent injection portion which injects a reagent into the first channel, and a nucleic acid pretreatment region which is disposed in the first channel and which performs pretreatment for the detection of a nucleic acid. The first channel, the second channel, the nucleic acid detection region, the nucleic acid pretreatment region, and the reagent injection portion are sealed.
A nucleic acid detection cassette comprising:
Dependencies are inferred from the claim text.
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From the US filing history. Foreign family members aren’t listed.
By integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g.…
By integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g.…
Fluid interfacing between devices or objects, e.g. connectors, inlet details: for microfluidic devices
Solutions for specific problems relating to chemical or physical laboratory apparatus: exchange or ejection of cartridges, containers or reservoirs
Term ended 19 Aug 2026 (estimated)
1. Field of the Invention
The present invention relates to a nucleic acid detection cassette which completely automatically performs the detection of a nucleic acid and its pretreatment step for a purpose of detecting a target nucleic acid, and a nucleic acid detection device by use of this nucleic acid detection cassette.
2. Description of the Related Art
FIG. 1 is an exploded perspective view schematically showing the whole constitution of a nucleic acid detection cassette in a first embodiment of the present invention;
FIG. 2 is a conceptual diagram of a section of the nucleic acid detection cassette in the first embodiment;
FIG. 3 is a diagram showing details of the section of the nucleic acid detection cassette in the first embodiment;
Embodiments will be described hereinafter with reference to the drawings.
FIG. 1 is a schematic perspective view of a nucleic acid detection cassette 100 in a first embodiment of the present invention. FIG. 2 is a conceptual diagram of a section of the nucleic acid detection cassette 100 of FIG. 1.
The nucleic acid detection cassette 100 includes a cassette upper body 1, an elastic sheet 3, and a cassette lower body 2. The elastic sheet 3 is sandwiched between the cassette upper body 1 and the cassette lower body 2 to thereby form the nucleic acid detection cassette 100. At this time, the sheet is sandwiched with an appropriate pressure to thereby keep sealability in the nucleic acid detection cassette 100. The cassette upper body 1 has a channel 11 on its inner surface, that is, the surface of the body which is brought into contact with the elastic sheet 3. The cassette lower body 2 has a groove 21 on its inner surface, that is, the surface of the body which is brought into contact with the elastic sheet 3. The channel 11 is connected to the groove 21 through a hole formed in the elastic sheet 3. The elastic sheet 3 may be provided with a groove forming a channel. A shape of the groove 21 is not especially limited, but examples of the shape of a section of the groove include a square shape, a rectangular shape, a semicircular shape, and a shape obtained by combining these shapes. Examples of a material of the cassette upper body 1 and the cassette lower body 2 include resins such as polyethylene, polypropylene, polystyrene, and polycarbonate, but the material is not especially limited to them. Examples of a material of the elastic sheet 3 include a resin such as silicon rubber, but the material is not especially limited to the example.