Multichannel rapid sampling of chromatographic peaks by tandem mass spectrometer
AssigneeAgilent Technologies, Inc.
InventorsGregor Overney, William Frazer, Harry Bunting, Chiachen Chang
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Abstract
Systems and methods for optimizing the analysis of co-eluting compounds during a cycle of a tandem mass spectrometer system are provided. The tandem mass spectrometer system switches fast from analyzing one compound ion to analyzing another compound ion and from one collision cell energy to another. The fast switching allows complex sampling patterns that improve coverage of the ionic signal of the co-eluting compounds while allowing different collision cell energies to be analyzed.
Claims32
A method of analyzing two or more compound ions during an analysis cycle of a tandem mass spectrometer system, the method comprising:
- analyzing the compound ions during a plurality of sets of analysis sub-cycles, wherein each of the compound ions is analyzed during each of said sets of sub-cycles, wherein the sub-cycles of a set occur consecutively in time, and wherein each sub-cycle uses a fixed collision cell energy, wherein:
- a first compound ion is analyzed during a sub-cycle of a first set of sub-cycles using a first collision cell energy;
- and
- a second compound ion is analyzed during another sub-cycle of said first set of sub-cycles using said first collision cell energy;
- and
- the first compound ion is analyzed during a sub-cycle of a second set of sub-cycles using a second collision cell energy different than the first collision cell energy the second compound ion is analyzed during another sub-cycle of said second set of sub-cycles using said second collision cell energy.
An information storage medium that stores a plurality of instructions adapted to direct an information processing device to provide control signals to a tandem mass spectrometer to perform an operation of analyzing two or more compound ions during an analysis cycle of the tandem mass spectrometer system, the operation comprising the steps of:
- analyzing the compound ions during a plurality of sets of analysis sub-cycles, wherein each of the compound ions is analyzed during each of said sets of sub-cycles, wherein the sub-cycles of a set occur consecutively in time, and wherein each sub-cycle uses a fixed collision cell energy, wherein:
- a first compound ion is analyzed during a sub-cycle of a first set using a first collision cell energy;
- and
- a second compound ion is analyzed during another sub-cycle of said first set of sub-cycles using said first collision cell energy;
- and
- the first compound ion is analyzed during a sub-cycle of a second set analyzes using a second collision cell energy different than the first collision cell energy the second compound ion is analyzed during another sub-cycle of said second set of sub-cycles using said second collision cell energy.
A tandem mass spectrometer system for analyzing two or more compound ions during an analysis cycle, the system comprising:
- a tandem mass spectrometer including a first mass analyzer, a collision cell, and a second mass analyzer;
- and
- a control system including:
- a processor for determining parameters for a cycle of the tandem mass spectrometer, wherein the parameters include a number of sets of analysis sub-cycles, a number of sub-cycles for each set, a compound ion to be analyzed for each sub-cycle, a number of transients for each sub-cycle, and the collision cell energy for each sub-cycle;
- and
- a controller for providing control signals to the tandem mass spectrometer based on the parameters, wherein the signals control the analysis of the compound ions,
- wherein the control signals control the mass spectrometer to analyze each of the compound ions during each of said sets of sub-cycles, wherein the sub-cycles of a set occur consecutively in time, and each sub-cycle uses a fixed collision cell energy, and wherein:
- a first compound ion is analyzed during a sub-cycle of a first set using a first collision cell energy;
- and
- a second compound ion is analyzed during another sub-cycle of said first set of sub-cycles using said first collision cell energy;
- and
- the first compound ion is analyzed during a sub-cycle of a second set using a second collision cell energy different than the first collision cell energy the second compound ion is analyzed during another sub-cycle of said second set of sub-cycles using said second collision cell energy.
Dependencies are inferred from the claim text.