DNA threading intercalators
AssigneeAgency for Science, Technology and Research
InventorsZhiqiang Gao, Fang Xie
Life of this patent
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Abstract
The invention is directed to a compound having the general formula (I): wherein each of La and Lb is an independently selected from a linking moiety comprising 0 to 10 main chain atoms, optionally substituted; each of Za and Zb is an independently selected complexing moiety comprising at least one nitrogen atom; either both or one of Za and/or Zb is coordinatively bonded to a respective transition metal complex MaVa and MbVb through said nitrogen atom, wherein each of Ma and Mb is an independently selected transition metal, and each of Va and Vb is an independently selected valence group.
Claims17
A compound having the general formula (I):
- wherein
- each of La and Lb is an independently selected aliphatic saturated hydrocarbon chain comprising 0 to 10 main chain atoms, optionally substituted;
- Za is an imidazole group, or a morpholine group
- Zb is an imidazole group or a morpholine group;
- and
- said imidazole group or morpholine group is coordinatively bonded to a respective transition metal complex MaVa, wherein
- the last main chain atom of La is covalently bonded to the imidazole group or morpholine group and the last main chain atom of Lb is covalently bonded to the morpholine group, or the imidazole group and wherein
- Ma is a transition metal, and
- Va is a valence group.
A method for producing a compound having the general formula (I):
- wherein
- each of La and Lb is an independently selected aliphatic saturated hydrocarbon comprising 0 to 10 main chain atoms, optionally substituted;
- Za is an imidazole group, or a morpholine group and
- Zb is an imidazole group or a morpholine group;
- said imidazole group or a morpholine group Za is coordinatively bonded to a respective transition metal complex MaVa, wherein
- Ma is a transition metal, and
- Va is a valence group,
- said process comprising:
- reacting 1,4,5,8-naphthalene tetracarboxylic dianhydride with a primary amine having the formula (IV):
- and/or a primary amine having the formula (V):
- thereby forming a naphthalene diimide intermediate;
- and
- reacting the naphthalene diimide intermediate with a transition metal complex, thereby coordinatively bonding the metal complex to the at least one nitrogen atom in Za.
A method of detecting a nucleic acid, comprising:
- contacting at least one capture probe immobilised on a substrate with the nucleic acid, said capture probe having a nucleotide sequence complementary to the sample, thereby hybridising the nucleic acid to the capture probe to form a hybrid;
- contacting the hybrid with a DNA threading intercalator compound having the general formula (I):
- wherein
- each of La and Lb is an independently selected aliphatic saturated hydrocarbon chain comprising 0 to 10 main chain atoms, optionally substituted;
- Za is an imidazole group, or a morpholine group
- Zb is an imidazole group or a morpholine group;
- and
- said imidazole group Za or morpholine group is coordinatively bonded to a respective transition metal complex MaVa wherein
- Ma is a transition metal, and
- Va is a valence group,
- thereby intercalating the hybrid;
- contacting an organic acid with the intercalated hybrid;
- applying an oxidising or reducing potential to the organic acid;
- and
- determining the electro chemical response of the organic acid.
A compound having the general formula (ii):
- wherein
- each of La and Lb is an independently selected aliphatic saturated hydrocarbon comprising 0 to 10 main chain atoms, optionally substituted, and
- Za is an imidazole group, and
- Zb is a morpholine group.
Dependencies are inferred from the claim text.