Quantitative analysis of a biological sample of unknown quantity
AssigneeHome Access Health Corporation
InventorsMichael Wandell, Ilia Vinogradov
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Abstract
Disclosed is a method for testing a modified specimen such as a dried blood spot, plasma or serum specimen, for an analyte of interest, such as cholesterol. In accordance with the disclosed subject matter, the level of the analyte of interest in the medium from which the modified specimen was obtained (e.g., from a patient's blood) is determined based on the level of an analyte in a solution formed from the modified specimen and on the level of at least one normalizing analyte. The analyte and normalizing analyte each may be an ion, compound, biochemical entity, or property of the specimen. Also disclosed are a fluid collector and a fluid collection device.
Claims22
A method for determining a global level of an analyte in blood from a solution formed from a dried blood fluid specimen eluted with a fluid, the blood fluid specimen being a plasma or serum specimen, comprising in either order:
- measuring a local level of said analyte in said solution and measuring the level of a first normalizing analyte and a second normalizing analyte;
- calculating a first blood analyte level based on said local level of said analyte and said level of said first normalizing analyte in said solution;
- calculating a second blood analyte level based on said local level of said analyte and said level of said second normalizing analyte in said solution;
- and
- calculating the global level of analyte in blood from which said blood fluid specimen was collected by calculating the weighted average of said first and second blood analyte levels.
A method for determining the concentration of an analyte in blood from a solution formed from a dried blood fluid specimen eluted with a fluid, and comprising:
- in any order, measuring the level of an analyte in said solution, measuring the level of a first normalizing analyte in said solution, and measuring the level of a second normalizing analyte in said solution;
- and
- calculating the concentration of the analyte level in the blood from which said blood fluid specimen was collected based on said analyte level in said solution, the level of said first normalizing analyte in said solution, and the level of said second normalizing analyte in said solution by calculating the weighted average of the level said first and second normalizing analyte in said solution.
A method for preparing a database for use in determining an analyte level in blood from a solution formed from a dried blood fluid specimen eluded with a fluid, said blood fluid specimen being a plasma or serum specimen comprising the measurement of first and second normalizing analytes in said solution, comprising:
- providing a plurality of blood samples each having a known blood analyte level;
- for each of said blood samples, preparing a blood fluid specimen thereby yielding a plurality of blood fluid specimens;
- for each of said blood fluid specimens, forming a solution;
- for each of said solutions, measuring the levels of said analyte, a first normalizing analyte, and a second normalizing analyte in said solution;
- and
- recording in a database record the known blood analyte level, the level of analyte measured in said solution, the level of first normalizing analyte measured in said solution and the level of said second normalizing analyte measured in said solution.
Dependencies are inferred from the claim text.