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US 7,479,379 B2Granted
Methods for altering protein production rates
AssigneeCentocor, Inc.
InventorsMichael Rycyzyn, Gregory Bannish, Jill Giles-Komar
Life of this patent
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Abstract
Methods for altering the cellular secretion rate of a protein, such as an antibody and the altered cells produced by the method are disclosed. The methods and altered cells are useful for producing high levels of proteins for therapeutic, diagnostic or research purposes.
Claims6
A method for increasing the cellular secretion rate of a protein comprising the steps of:
- a) decreasing the activity of an unfolded protein response (UPR) pathway component in a cell by stably transfecting the cell with a nucleic acid encoding a siRNA that targets transcripts of a nucleic acid sequence encoding a CHOP protein, wherein the nucleic acid encoding the siRNA is the nucleic acid sequence of SEQ ID NO:
- 15 or SEQ ID NO:
- 16;
- and b) culturing the cells.
1 more element
Dependencies are inferred from the claim text.