US 7,479,367 B1 · In vivo assay and molecular markers for testing the phenotypic stability of cell populations and selected cell populations for autologous transplantation

Abstract

An in vivo assay to measure anchorage-independent growth and phenotypic stability of a certain cell population comprising subcutaneous or intramuscular injection in a mammal of a cell suspension of articular chondrocytes in an iso-osmotic liquid, the same suspension comprising articular chondrocytes in an amount equivalent to at least 1×106 chondrocytes as applied to immune-deficient mice. The outcome is linked to molecular markers. The present invention further relates to DNA chips and diagnostic tools comprising the latter to predict the outcome of ACT. Antibodies raised against positive and negative markers of chondrocyte stability can also be used for quality control on the chondrocytes. Therapeutical composition comprising stable chondrocytes are very useful for tissue repair.

Claims15

Dependent claims

A method to identify chondrocyte cells having chondrocyte phenotypic stability comprising:

(1) determining the expression by said cells of a positive marker of chondrocyte phenotypic stability which is bone morphogenic protein-2 (BMP-2) and/or determining the expression by said cells of a positive marker of chondrocyte phenotypic stability which is fibroblast growth factor receptor 3 (FGFR-3) and the absence of expression of a negative marker which is activin-like kinase-1 (ALK-1);
and
(2) identifying said cells expressing said BMP-2 and/or said cells expressing FGFR-3 and not said ALK-1, as cells having chondrocyte phenotypic stability.

Dependencies are inferred from the claim text.

Facts

Application

10/089,932Filed 6 Oct 2000

Priority

EP 992032736 Oct 1999

Granted

20 Jan 2009

Term ends

Not estimatedThe source records don’t support an estimate.

Classification8 codes

A61K 49/0006
Screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance, gastric emptying, testing for…: skin…
A61K 49/0004
Preparations for testing in vivo: screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance,…
A61K 49/0008
Screening or testing of compounds for diagnosis of disorders, assessment of conditions, e.g. renal clearance, gastric emptying, testing for…:…
A61P 19/04
Drugs for skeletal disorders: for non-specific disorders of the connective tissue

In vivo assay and molecular markers for testing the phenotypic stability of cell populations and selected cell populations for autologous transplantation

Life of this patent

In vivo assay and molecular markers for testing the phenotypic stability of cell populations and selected cell populations for autologous transplantation

Life of this patent

Abstract

Claims15