Process for preparing oligonucleotides
AssigneeAvecia Biotechnology Inc.
InventorsDavid John Moody, Donald Alfred Wellings, Paul McCormac
Life of this patent
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Abstract
A process for the preparation of an oligonucleotide is provided. The process comprises the assembly of an oligonucleotide attached to a solid support, wherein the solid support is prepared by a process comprising polymerisation of a monomer which comprises a protected hydroxypolyC2-4 alkyleneoxy chain attached to a polymerisable unit wherein the protected hydroxypolyC2-4 alkyleneoxy chain contains from 2 to 10 C2-4 alkyleneoxy groups and wherein the hydroxypolyC2-4 alkyleneoxy chain is protected with an acid-labile protecting group, preferably an optionally substituted trityl group.
Claims15
In a process for the preparation of an oligonucleotide by assembly of the oligonucleotide attached to a solid support, the improvement wherein the solid support is prepared by polymerisation of a monomer which comprises a protected hydroxypolyC2-4 alkyleneoxy chain attached to a polymerisable unit wherein the protected hydroxypolyC2-4 alkyleneoxy chain contains from 2 to 10 C2-4 alkyleneoxy groups and wherein the hydroxypolyC2-4 alkyleneoxy chain is protected with a poly-aryl methane protecting group.
A composition of matter having the formula:
- Ps-Z-Q
- wherein
- Ps represents a polymer obtained by polymerisation of a monomer which comprises a protected hydroxypolyC2-4 alkyleneoxy chain attached to a polymerisable unit wherein the protected hydroxypolyC2-4 alkyleneoxy chain contains from 2 to 10 C2-4 alkyleneoxy groups and is protected with a poly-aryl methane protecting group;
- Z represents a single bond or a cleavable linker;
- and
- Q represents H, a nucleoside or an oligonucleotide, provided that Q is not H when Z represents a single bond.
Dependencies are inferred from the claim text.