Back to results
US 7,476,503 B2Granted
Apparatus and method for performing nucleic acid analysis
AssigneePacific Biosciences of California, Inc.
InventorsStephen Turner, Jonas Korlach
Life of this patent
Estimating the term…
Abstract
The present invention relates to optical confinements, methods of preparing and methods of using them for analyzing molecules and/or monitoring chemical reactions. The apparatus and methods embodied in the present invention are particularly useful for high-throughput and low-cost single-molecular analysis.
Claims30
A nucleic acid sequencing method comprising:
- subjecting a target nucleic acid molecule to a template-directed polymerization reaction to yield a nascent nucleic acid strand that is complementary to the target nucleic acid molecule in the presence of a plurality of types of nucleotides or nucleotide analogs, and a polymerization enzyme exhibiting strand-displacement activity, wherein said template-directed polymerization reaction processes an identical sequence of nucleotides in the target nucleic acid molecule more than once;
- registering a time sequence of incorporation of nucleotides or nucleotide analogs into the nascent nucleotide strand;
- and determining a sequence of nucleotides in the target sequence.
Dependencies are inferred from the claim text.