# US 7,465,581 B2 · ANKTM1, a cold-activated TRP-like channel expressed in nociceptive neurons

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- Number: US 7,465,581 B2
- Document ID: US7465581B2
- Status: Granted
- Kind: B2
- Application number: 10/539,377
- Filed: 2003-12-17
- Published: 2006-06-29
- Granted: 2008-12-16
- Earliest claimed: 2002-12-18
- Assignees: The Scripps Research Institute; Novartis AG
- Inventors: Stuart Bevan; Ardem Patapoutian; Gina M. Story
- Web page: https://patentgrep.com/patent/US7465581B2

## CPC

- C07K 14/705: Receptors; Cell surface antigens; Cell surface determinants {(tumour specific antigens C07K14/4748)}
- A61P 25/00: Drugs for disorders of the nervous system
- G01N 33/5088: {of vertebrates}
- A01K 2217/05: Animals comprising random inserted nucleic acids (transgenic)
- A01K 2217/075: inducing loss of function, i.e. knock out
- A01K 2227/10: Mammal
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- A61P: SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- G01N: INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES (measuring or testing apparatus or processes other than immunoassay, involving enzymes or microorganisms C12M, C12Q)
- A01K: ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS

## Abstract

The methods and compositions of the invention are based on a method for measuring nociceptive responses in vertebrates, including humans and other mammals utilizing a newly discovered thermoreceptor belonging to the Transient Receptor Potential (TRP) family of non-selective cation channels that participates in thermosensation and pain. This receptor, designated ANKTMI, is associated with nociceptive pain, such as hyperalgesia. Accordingly, the invention provides isolated polypeptides and polynucleotides associated with nociception as well as methods for identifying or screening agents that modulate nociception.

## Claims

### Claim 1

An isolated nucleic acid encoding an ANKTM1-related polypeptide consisting of the amino acid sequence as set forth in SEQ ID NO: 2.

### Claim 2 (depends on 1)

An expression vector comprising the nucleic acid of claim 1.

### Claim 3 (depends on 2)

The vector of claim 2, wherein the vector is a plasmid.

### Claim 4 (depends on 2)

The vector of claim 2, wherein the vector is a virus.

### Claim 5 (depends on 2)

An isolated host cell stably transformed with the vector of claim 2.

### Claim 6 (depends on 5)

The host cell of claim 5, wherein the cell is prokaryotic.

### Claim 7 (depends on 5)

The host cell of claim 5, wherein the cell is eukaryotic.