Metabolically engineered lactic acid bacteria and their use
OwnerDan Nilssoninventor
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Abstract
Mutants of lactic acid bacteria including Lactococcus lactis which are defective in pyruvate formate-lyase production and/or in their lactate dehydrogenase (Ldh) production and methods of isolating such mutants or variants are provided. The mutants are useful in the production of food products or in the manufacturing of compounds such as diacetyl, acetoin and acetaldehyde and as components of food starter cultures.
Claims6
A lactic acid bacterium which is selected from the group consisting of Lactococcus lactis subspecies lactis strain DN221 deposited under the accession No. DSM 11034, a strain essentially having the characteristics of DSM 11034, Lactococcus lactis subspecies lactis biovar diacetylactis strain DN227 deposited under the accession No. DSM 11040 and a strain essentially having the characteristics of DSM 11040.
A pyruvate formate-lyase (Pfl) defective lactic acid bacterium which is
- (I) Lactococcus lactis subspecies lactis strain DN221 deposited under the accession number DSM 11034, or
- (II) Lactococcus lactis subspecies lactis strain DN227 deposited under the accession number DSM 11040.
A lactic acid bacterium which is Lactococcus lactis subspecies lactis strain DN225, deposited under the accession number DSM11038, or a strain having all of the characteristics of DSM 11038.
Dependencies are inferred from the claim text.