# US 7,465,464 B2 · Populations of cells that express flk-2 receptors

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- Number: US 7,465,464 B2
- Document ID: US7465464B2
- Status: Granted
- Kind: B2
- Application number: 11/030,539
- Filed: 2005-01-05
- Published: 2005-08-11
- Granted: 2008-12-16
- Earliest claimed: 1991-04-02
- Assignees: The Trustees of Princeton University
- Inventors: Ihor R. Lemischka
- Web page: https://patentgrep.com/patent/US7465464B2

## CPC

- C07K 14/52: Cytokines; Lymphokines; Interferons
- C07K 14/47: from mammals
- C07K 14/705: Receptors; Cell surface antigens; Cell surface determinants {(tumour specific antigens C07K14/4748)}
- C07K 14/70596: {Molecules with a "CD"-designation not provided for elsewhere}
- C07K 14/71: for growth factors; for growth regulators
- C07K 14/715: for cytokines; for lymphokines; for interferons
- C07K 16/28: against receptors, cell surface antigens or cell surface determinants
- C12N 5/0647: {Haematopoietic stem cells; Uncommitted or multipotent progenitors}
- C12N 9/1205: {Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases}
- C07K 2319/00: Fusion polypeptide
- C07K 2319/32: fusions with soluble part of a cell surface receptor, "decoy receptors"
- C07K 2319/43: containing a FLAG-tag
- C07K 2319/61: containing an enzyme fusion for detection (lacZ, luciferase)
- Y10S 435/971: Capture of complex after antigen-antibody reaction
- C07K: PEPTIDES (peptides in foodstuffs A23; obtaining protein compositions for foodstuffs, working-up proteins for foodstuffs A23J; preparations for medicinal purposes A61K; peptides containing beta-lactam rings C07D; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, C07D; ergot alkaloids of the cyclic peptide type C07D519/02; macromolecular compounds having statistically distributed amino acid units in their molecules, i.e. when the preparation does not provide for a specific; but for a random sequence of the amino acid units, homopolyamides and block copolyamides derived from amino acids C08G69/00; macromolecular products derived from proteins C08H1/00; preparation of glue or gelatine C09H; single cell proteins, enzymes C12N; genetic engineering processes for obtaining peptides C12N15/00; compositions for measuring or testing processes involving enzymes C12Q; investigation or analysis of biological material G01N33/00)
- C12N: MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA (microbiological testing media C12Q1/00)
- Y10S: TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS

## Abstract

Isolated mammalian nucleic acid molecules encoding receptor protein tyrosine kinases expressed in primitive hematopoietic cells and not expressed in mature hematopoietic cells are provided. Also included are the receptors encoded by such nucleic acid molecules; the nucleic acid molecules encoding receptor protein tyrosine kinases having the sequences shown in FIG. 1a (murine flk-2), FIG. 1b (human flk-2) and FIG. 2 (murine flk-1); the receptor protein tyrosine kinases having the amino acid sequences shown in FIG. 1a, FIG. 1b and FIG. 2; ligands for the receptors; nucleic acid sequences that encode the ligands; and methods of stimulating the proliferation and/or differentiation of primitive mammalian hematopoietic stem cells comprising contacting the stem cells with a ligand that binds to a receptor protein tyrosine kinase expressed in primitive mammalian hematopoietic cells and not expressed in mature hematopoietic cells.

## Claims

### Claim 1

An isolated population of cells that express on their surface a receptor that is a FLK-2 protein, wherein the sequence of the FLK-2 protein is SEQ. ID. NO: 4, wherein the population is isolated by a method comprising binding the cells to a polyclonal or monoclonal antibody specific for the FLK-2 receptor, and isolating the cells that are bound to the antibody from cells that are unbound to the antibody, wherein said isolated population of cells is bound to said antibody.